Plant Transcription Factor Database
Previous version: v3.0
Oryza barthii
HSF Family
Species TF ID Description
OBART01G20060.1HSF family protein
OBART01G23320.1HSF family protein
OBART01G29920.1HSF family protein
OBART01G30880.1HSF family protein
OBART02G17360.1HSF family protein
OBART02G18800.1HSF family protein
OBART02G33140.1HSF family protein
OBART03G04630.1HSF family protein
OBART03G08980.1HSF family protein
OBART03G33860.1HSF family protein
OBART03G33860.2HSF family protein
OBART03G33860.3HSF family protein
OBART03G37290.1HSF family protein
OBART03G41440.1HSF family protein
OBART04G22030.1HSF family protein
OBART05G23630.1HSF family protein
OBART06G18200.1HSF family protein
OBART06G18790.1HSF family protein
OBART07G04960.1HSF family protein
OBART07G23790.1HSF family protein
OBART08G17760.1HSF family protein
OBART08G22550.1HSF family protein
OBART09G12650.1HSF family protein
OBART09G12750.1HSF family protein
OBART10G10150.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645